Figure 6

Intracellular butyrate results in lower TRPM6/7 channel activity. (A) ²⁵Mg²⁺ uptake by Caco-2 cells after treatment with 8 mmol/L Na-butyrate and/or 30 µmol/L NS8593 after 10 min. Data represent mean ± SEM (n = 3, each experiment consisted of triplicates). * P < 0.05 is considered statistically significant compared to control cells using One-Way ANOVA with Sidak’s correction for multiple testing. (B, C) Whole-cell currents measured at − 80 mV and + 80 mV over time in TRPM6/7-transfected (HA-mTRPM7 pCINeo IRES GFP and HA-hTRPM6 pCINeo IRES GFP) HEK293 cells without (circles, n = 9) or with 8 mmol/L Na-butyrate (squares, n = 11) in the intracellular pipette solution (B). Bar graphs of current amplitudes without (Control) or with 8 mmol/L Na-butyrate (Na-butyrate) in the intracellular pipette solution (C). Data represent mean ± SEM. *P < 0.05 is considered statistically significant compared to control cells using Student’s t-test.