Figure 1
From: Neuromedin U-deficient rats do not lose body weight or food intake
Generation of NMU-deficient rats. (A) Schematic diagram of the target sequence, PAM, and ssDNA at the Nmu gene locus. (B) Genome sequence of the mutation in the Nmu mutant allele. (C) Efficiencies of rat Nmu gene editing by the rGONAD method. (D) Genotyping of Hetero, WT, and KO performed by PCR. A representative gel image of genomic PCR products amplified from the indicated genotypes is shown. (E) Immunostaining of NMU in WT and NMU KO rat. The light micrograph is representative of IHC staining with the anti-NMU antibody in a frontal section of the brain. Positive signals are indicated by arrows. Scale bar: 50 µm. PT, pars tuberalis; ME, median eminence. See also Supplementary Figure S1.
