Figure 3
From: Site-specific CRISPR-based mitochondrial DNA manipulation is limited by gRNA import
Delivery of base editor Cas9-BE3 into mitochondria. (A) Schematic outline of Cas9-BE3 activity. (B) Outline of mitochondria-targeted Cas9-BE3. (C) Confocal microscopy showing colocalization of FLAG-tagged Cas9-BE3 with mitochondria in HEK 293T cells. Scale bars: 10 μm. (D) Frequency of C to T conversions in HEK 293T cells treated with different constructs. Selected part of site within mtDNA that was targeted by mtORI gRNAs is shown. Only successfully transfected, GFP + cells were sorted and analyzed with amplicon next generation sequencing.
