Figure 2 | Scientific Reports

Figure 2

From: Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

Figure 2

EDN2 and EDNRB expression levels were notably up-regulated in samples of liver tissues and sera of PBC patients relative to healthy control group. (A and B) RNA-seq outcomes of EDN1, EDNRA, EDN2, and EDNRB in liver tissues of patients with PBC (n = 5) and healthy volunteers (n = 5). (C and D) RT-qPCR technique was used to measure levels of EDN1, EDNRA, EDN2, and EDNRB mRNAs in liver tissues of patients with PBC (n = 5) and healthy volunteers (n = 5). (E) Determination of EDN1 and EDN2 mRNAs in sera of PBC patients (n = 10) and healthy volunteers (n = 10) was accomplished with RT-qPCR technique. (F) Measurement of EDN1, EDNRA, EDN2, and EDNRB proteins in liver tissues of patients with PBC (n = 5) and healthy volunteers (n = 5) was carried out with western blotting technique. (G) We examined the protein levels of EDN1 and EDN2 in serum samples of 5 random PBC patients and 5 random healthy volunteers with western blotting technique. (H) Illustrative IHC images of EDNRA and EDNRB in liver tissues of healthy people and patients with PBC.

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