Figure 3 | Scientific Reports

Figure 3

From: Testis-expressed gene 11 inhibits cisplatin-induced DNA damage and contributes to chemoresistance in testicular germ cell tumor

Figure 3

Effects of TEX11 expression on cisplatin-induced apoptosis and DNA double-strand break (DSB) marker γH2AX expression in TGCT cells. (a, b) Immunoblotting of cleaved PARP1 in cisplatin-resistant TGCT-PDC-R (a) and NEC8-R (b) cells transfected with control (siControl) or TEX11-specific siRNAs (siTEX11 #1 and #2), without or with cisplatin treatment 48 h after siRNA transfection. β-actin was used as a loading control. (c) Representative γH2AX immunohistochemical staining in TGCT-PDC-R cells transfected with indicated siRNAs, followed by cisplatin treatment 6 h after siRNA transfection. (d) Percentages of γH2AX-positive cells among the examined TGCT-PDC-R cells treated with the indicated siRNAs. The results are shown as mean percentage ± SE. (e) Representative γH2AX immunohistochemical staining in NEC8-R cells transfected with indicated siRNAs in the presence of cisplatin. (f) Percentages of γH2AX-positive cells among the examined NEC8-R cells treated with the indicated siRNAs. *, P < 0.05.

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