Figure 4 | Scientific Reports

Figure 4

From: Testis-expressed gene 11 inhibits cisplatin-induced DNA damage and contributes to chemoresistance in testicular germ cell tumor

Figure 4

TEX11-specific siRNA injection significantly represses the development of TGCT-PDC-R-derived xenograft tumors in severely immunodeficient mice treated with cisplatin. (a) Volumes of tumors generated by TGCT-PDC-R cells in 7-week male NOD/SCID mice treated with intratumoral injection of control (siControl) or TEX11-specific (siTEX11) siRNAs plus intraperitoneal cisplatin administration. (b)Weights of tumors generated by TGCT-PDC-R in mice treated with injection of indicated siRNAs plus cisplatin administration. (c) Body weights of tumor-bearing mice treated with injection of indicated siRNAs plus cisplatin administration. Results are shown as mean ± SE. **, P < 0.01. (d) Images of dissected tumors at the endpoint. (e) Representative images of tumor-bearing mice at the endpoint. (f) Immunoblotting of TEX11 and cleaved PARP1 in dissected TGCT-PDC-R tumors. β-actin was used as a loading control. (g) Relative expression of TEX11 and cleaved PARP1 normalized to β-actin expression analyzed by densitometry. Results are shown as mean ± SE (n = 5). *, P < 0.05, **, P < 0.01.

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