Figure 1
From: Diminazene aceturate attenuates hepatic ischemia/reperfusion injury in mice
Effect of DIZE on liver injury following hepatic ischemia/reperfusion. C57BL/6 male mice were subjected to partial hepatic ischemia (HIR) induction for 1 h or sham surgery. Mice were randomized to receive treatment with either Diminazene aceturate (DIZE) or vehicle control (saline) before undergoing surgery, as described in “Methods”. (A) Four and twenty-four hours after surgeries, plasma ALT levels were measured. Liver sections were subjected to hematoxylin and eosin (H&E) staining (B, Upper panel: magnification X200; lower panel: magnification X400) and histological damages (C, D, E, and F) were analyzed using Suzuki’s criteria (sinusoidal congestion, parenchymal cell necrosis and cytoplasmic vacuolization). Results are expressed as mean ± SEM (n = 5). For statistical analysis, the one-way ANOVA plus Tukey’s post hoc multiple comparison test was used to detect significant changes in plasma ALT levels and the Mann–Whitney nonparametric test and t test were used to detect significant changes of Suzuki score with Origin 2021 v.9.8.0 200 (OriginLab, https://www.originlab.com) software. *P < 0.05 vs. Vehicle Sham, †P < 0.05 vs. Vehicle HIR.
