Figure 2
From: Diminazene aceturate attenuates hepatic ischemia/reperfusion injury in mice
Effect of DIZE on apoptosis following hepatic ischemia/reperfusion. C57BL/6 male mice were subjected to partial hepatic ischemia (HIR) induction for 1 h or sham surgery. Mice were treated with either Diminazene aceturate (DIZE) or vehicle control (saline) before undergoing surgery, as described in “Methods”. Twenty-four hours after surgery, liver sections were subjected to TUNEL staining (A; magnification X200) and the TUNEL-positive cells were counted (B). (C) Whole liver lysates were subjected to western blotting using B-cell lymphoma 2 (Bcl-2) and cleaved caspase-3 antibodies. GAPDH was used as a loading control. (D, E) Band intensities were measured using the ImageJ v.1.53e software. Results are expressed as mean ± SEM (n = 5). For statistical analysis, one-way ANOVA with plus Tukey’s post hoc multiple comparison test were used to detect significant changes with Origin 2021 v.9.8.0 200 (OriginLab, https://www.originlab.com) software. *P < 0.05 vs. Vehicle Sham, †P < 0.05 vs. Vehicle HIR.
