Table 1 Primers used in quantitative reverse transcription polymerase chain reactions to amplify mouse cDNAs based on published GenBank sequences.
From: Diminazene aceturate attenuates hepatic ischemia/reperfusion injury in mice
Primers | Sequence (sense/antisense) | Annealing temp (°C) |
|---|---|---|
mouse TNF-α | 5′-TACTGAACTTCGGGGTGATTGGTCC-3′ | 65 °C |
5′-CAGCCTTGTCCCTTGAAGAGAACC-3′ | ||
mouse MCP-1 | 5′-ACCTGCTGCTACTCATTCAC-3′ | 60 °C |
5′-TTGAGGTGGTTGTGGAAAAG-3′ | ||
mouse MIP-2 | 5′-CCAAGGGTTGACTTCAAGAAC-3′ | 60 °C |
5′-AGCGAGGCACATCAGGTACG-3′ | ||
mouse KC | 5′-CAATGAGCTGCGCTGTCAGTG-3′ | 60 °C |
5′-CTTGGGGACACCTTTTAGCATC-3′ | ||
mouse IL-6 | 5′-CCGGAGAGGAGACTTCACAG-3′ | 62°C |
5′-GGAAATTGGGGTAGGAAGGA-3′ | ||
mouse IL-1β | 5′-CTGAAAGCTCTCCACCTC-3′ | 56 °C |
5′-TGCTGATGTACCAGTTGGGG-3′ | ||
GAPDH | 5′-ACCACAGTCCATGCCATCAC-3′ | 65 °C |
5′-CACCACCCTGTTGCTGTAGCC-3′ |
