Figure 2 | Scientific Reports

Figure 2

From: Characterization of collagen response to bone fracture healing using polarization-SHG

Figure 2

PA maps and corresponding PA histograms for (a,b) tendon, (c,d) cortical bone (Zone A), (e,f) articular cartilage (Zone B) of healthy bone control sample, and (g,h) bone fracture repair at 2 weeks, and (i,j) bone fracture repair at 4 weeks. (k) Averaged PA values for tendon, articular cartilage (Zone B), cortical bone (Zone A), and fracture repair at 2 weeks, and fracture repair at 4 weeks. Error bars represent standard error of mean. Image size is 50 × 50 μm2. The tendon and Zone A (COL I) have lower pitch angle as compared to Zone B (COL II), as observed in the colormaps (a,c,e). Similar decrease in the pitch angle can be observed for the colormap of 4 weeks (i) rich in collagen type I as compared to 2 weeks (g). The mean values of the healing tissue follow similar trend as that of the control tissue, despite the healing tissues not being significantly different at p < 0.05. All the image maps, histograms, and bar graphs were generated by using MATLAB R2019a (Version: 9.6.0.1072779, https://www.mathworks.com/products/new_products/release2019a.html).

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