Figure 6 | Scientific Reports

Figure 6

From: Loss of stability and unfolding cooperativity in hPGK1 upon gradual structural perturbation of its N-terminal domain hydrophobic core

Figure 6

Conformational stability of WT and F25 mutants as determined by proteolysis with thermolysin. (A) Representative SDS-PAGE gels of hPGK1 variants proteolysis kinetics; (B) Densitometric analysis of results shown in panel A with fittings to a single exponential function (left panel) and the corresponding half-lives (right panel). Thermolysin concentration was 1 µM. Experiments were carried out in 20 mM HEPES–NaOH 200 mM NaCl, pH 7.4 at 25 °C.

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