Table 5 Best-fit parameters for selected segments of WT hPGK1 determined by HDXMS.

From: Loss of stability and unfolding cooperativity in hPGK1 upon gradual structural perturbation of its N-terminal domain hydrophobic core

Parameter

A∞ (%D)

A1 (%D)

k1 (s−1)

A2 (%D)

k2 (s−1)

r2

Segment

12

100.5 ± 1.8

45.1 ± 2.7

7.2 ± 1.1·10−3

48.9 ± 2.7

1.3 ± 0.2·10−4

0.9986

19–21

21.1 ± 0.7

19.6 ± 1.2

2.0 ± 0.4·10−2

4.2 ± 0.9

1.2 ± 0.7·10−4

0.9956

36

96.5 ± 0.3

16.9 ± 0.4

1.3 ± 0.1·10−2

17.0 ± 0.3

1.2 ± 0.1·10−4

0.9998

89–92

96.6 ± 3.7

26.4 ± 4.7

6.1 ± 2.9·10−3

31.7 ± 5.0

7.5 ± 3.2·10−5

0.9858

142–145

98.0 ± 1.0

28.1 ± 2.0

1.4 ± 0.3·10−2

9.2 ± 1.9

3.4 ± 1.7·10−4

0.9957

192–194*

97.8 ± 1.4

98.5 ± 1.5

6.5 ± 0.3·10−5

N.app

N.app

0.9990

213–216

76.4 ± 1.4

32.4 ± 1.9

5.8 ± 0.9·10−3

12.9 ± 2.0

9.3 ± 3.8·10−5

0.9968

244

32.2 ± 3.1

10.3 ± 0.5

8.1 ± 1.1·10−3

16.0 ± 3.0

1.8 ± 0.5·10−5

0.9986

296

94.9 ± 3.2

46.6 ± 4.7

5.7 ± 1.4·10−3

22.3 ± 4.8

1.2 ± 0.7·10−4

0.9931

348–353

48.7 ± 0.6

17.4 ± 1.1

1.0 ± 0.1·10−3

27.9 ± 1.0

4.8 ± 0.6·10−5

0.9997

381*

97.8 ± 0.8

41.1 ± 3.6

2.4 ± 0.5·10−2

N.app

N.app

0.9836

404–405

96.5 ± 3.3

30.2 ± 8.0

3.6 ± 1.8·10−2

57.8 ± 3.7

7.7 ± 1.4·10−5

0.9934

  1. Good fittings were obtained using a two-exponential function (A∞ is the total amplitude of %D uptaken at t = ∞, A1 and k1 are the amplitude and rate constant for the fast phase, while A2 and k2 are those for the slow phase).
  2. *The fitting to a double-exponential function did not converge. The values reported corresponded to a fitting using a one-exponential function with a burst-phase. N. app.- not applicable.
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