Figure 3

Involvement of amino acid transporters in the action of ferrichrome. (A) Growth of pub1Δ leu1-32 and any1Δ leu1-32 cells on EMM containing 2.0% NH₄Cl supplemented with leucine. A sample of 50% MeOH (3 μl) or ferrichrome (150 ng in 3 μl 50% MeOH) was spotted next to the yeast cell suspensions (denoted as ‘i’). Representative images from three independent experiments are shown. (B) Growth of leu1-32 and cat1Δ leu1-32 cells on EMM containing 2.0% NH₄Cl supplemented with leucine. Adaptive growth was observed as in A. Representative images from three independent experiments are shown. (C) Effect of cat1 deletion on the cell growth in liquid media. Cells were inoculated in EMM + 2.0% NH₄Cl supplemented with leucine. Data represent the mean ± SD (n = 3). (D) Effect of cat1 deletion on the growth recovery by ferrichrome. Cells were inoculated as in C for 48 h with a variety of concentrations of ferrichrome. Data represent the mean ± SD (n = 3–4).