Figure 1

Overlay of a representative region of the 2D 13C-1H HSQC spectra of a representative uninoculated BSF control (green; bottom), suspended culture (blue; middle), and biofilm (red; top) sample. Samples are all at 13C natural abundance and each shifted 0.01 ppm down-field (left) for better visualization. Unique peaks were detected between each sample indicating metabolic differences between the BSF control and P. aeruginosa cultures grown in BSF as suspended culture and biofilm phenotypes.