Figure 7

Metabolic pathway map displaying significant metabolite quantity differences between the suspended and lawn biofilm cultures. Metabolites with a fold change (FC) (biofilm/suspended) > 2 are red indicating higher levels in biofilm and FC < 0.5 are blue indicating higher levels in suspended culture. Pathways showing significant changes between suspended culture and biofilm have the arrows color-coded including alanine metabolism (purple), creatine metabolism (brown), glutathione metabolism (dark blue), choline metabolism (green), and the TCA cycle (orange). Metabolites that were detected but the fold change is less than two-fold are shown in black and metabolites not detected are shown in gray (GABA gamma-aminobutyric acid, aKG alpha-ketoglutarate, ATP adenosine triphosphate, ADP adenosine diphosphate, NAD nicotinamide adenine dinucleotide, NADH nicotinamide adenine dinucleotide reduced, GPC glycerophosphocholine, G-3-P glycerol-3-phosphate, PC phosphorylcholine, GPE glycerophosphoethanolamine).