Figure 4

Myofibroblast quantification of periarticular knee tissue. Myofibroblast quantification and representative immunofluorescence merged images for α-SMA (green), Laminin (red) and DAPI (blue) from the three patient groups (PTKA = primary TKA, RTKA-A = arthrofibrosis, RTKA-NA = non-arthrofibrosis). Sample 1, Sample 2, and Sample 3 depict images from three different patients for each group. The graphs depict mean myofibroblast number defined as ACTA2 + /Laminin- per area ± standard deviation, with each data point representing one patient. Two asterisks denote a significant difference, p ≤ 0.01, by the Wilcoxon Rank Sum test. The scale bar applies to all images in this figure.