Figure 4 | Scientific Reports

Figure 4

From: Generation and maturation of human iPSC-derived 3D organotypic cardiac microtissues in long-term culture

Figure 4

Long-term 3D culture of hOCMTs recapitulates advanced cardiac morphology and cellular heterogeneity in vitro showing markers for endocardial, endothelial, and cardiomyocyte subtypes. Immunofluorescence analysis showing the representative co-specification of multiple cell type markers in 3D hiPSCs-derived cardiac microtissue/hOCMT sections on day 50 including endocardial ([A,B] NFAT2 in green), endothelial cells ([B] CD31 in red), pan-cardiomyocytes ([A] ACTN2 in red), atrial ([C] MYL7 in green) and ventricular ([C] MYL2 in red) cardiomyocyte subtypes. Nuclei were counterstained with DAPI (blue). Full 3D hOCMTs scale bar = 200 µm, Close-up scale bar = 50 µm.

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