Figure 5
Ultrastructural analysis of 3D hOCMTs indicates enhanced maturation in 3D environment over time. (A) TEM images of 3D hOCMTs s showing increasing ultrastructural organization over time, from day 21 to day 85: Cardiomyocyte myofibers (Myo), Mitochondria (M) Z-band (Z), T-tubules. Scale bars = 200 nm. (B) Close-up images on day 50. Scale bars = 200 nm. (C) Representative micrograph showing sarcomere length (SL; white dotted two-headed arrow) and z-band width (ZW; cross sectional length; white solid two-headed arrow) with the corresponding sarcomere length (D); day 50, 1400 ± 300 nm; day 85, 1500 ± 200 nm; N > 20, Mean ± SD)- and z-band width (E); day 50, 450 ± 100 nm; day 85 700 ± 250 nm; N > 30, Mean ± SD). Scale bar = 200 nm. (F) Micrograph showing myofiber organization (white arrow) with their relative organization as defined by the plot profile intensity on day 50 vs day 85 (G) of the area indicated by the grey dashed box of (F). Scale bar = 200 nm.
