Figure 6 | Scientific Reports

Figure 6

From: Generation and maturation of human iPSC-derived 3D organotypic cardiac microtissues in long-term culture

Figure 6

3D long-term cultures induce improved cardiac specification and cardiomyocyte maturation at transcriptional level. (A) MA plot of the differentially regulated genes between 2D monolayer and 3D hOCMTs at day 30 (up) and day 50 (down) (log2 mean, p.adj < 0.05). (B) Graph representing the − log2value adjusted p-value of significantly upregulated (red) or downregulated (blue) GO Biological process categories when comparing 3D hOCMTs and 2D monolayers at day 30 (up) and day 50 (down) (p.adj < 0.05). (C) Heatmap representing the log2fold change for the top 20 differentially regulated genes between 2D monolayer and 3D hOCMTs at day 50 (p.adj < 0.05). (D) STRING network of the upregulated genes at day 50 hOCMTs as compared to monolayer cultures for the indicated GO biological processes categories by Cytoscape (Kappa score = 0.3). Log2fold change for the nodes (genes) is represented using a color-coded scale. (E) Heatmap encoding for log2fold changes in selected cardiac genes for the 3D hOCMTs and the 2D monolayers at both time-points in comparison with available datasets for adult human atrial and ventricular tissues (p.adj < 0.05).

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