Figure 7
3D long-term cultures promote changes in metabolic activity towards maturation. (A) Diagram showing metabolic genes with significantly upregulated (in red) and downregulated (in green) genes between long-term 3D hOCMTs vs 2D monolayer cultures (log2fold change; p.adj < 0.05). (B) Graphs for comparing the cellular energy metabolism of long-term 3D hOCMTs vs 2D monolayer cultures after day 50 using Seahorse XFp Analyzer and Seahorse Mito Stress Test Kit: (i) oxidative phosphorylation (oxygen consumption rate, OCR) and (ii) glycolytic flux (extracellular acidification rate, ECAR) (Mean ± SEM obtained in four independent analyses. Total sample size: n2D = 12, n3D = 9). (C) Bar graphs providing a detailed analysis of the oxygen consumption rate (OCR) Seahorse XFp Analyzer data presented in (B): (i) basal respiration and spare respiratory capacity, (ii) proton leak, ATP production (Mean ± SEM, N = 4, n2D = 12, n3D = 9, unpaired Student’s t test—two-tailed).
