Figure 8
Long-term 3D hOCMTs show functional response to cardioactive and cardiotoxic drugs in a dose and time dependent manner. (A) Representative contraction amplitude plots of long-term (> day 50) Long-term 3D hOCMTs in response to untreated vs increasing doses of Isoproterenol and Verapamil. (B) Average beating rates of 3D long-term cardiac microtissues hOCMTs in response to increasing doses of Isoproterenol and Verapamil (n = 3, Mean ± SD, Dunnett’s multiple comparisons test). (C) Morphology of 3D long-term (> day 50) Long-term 3D hOCMTs in response to untreated vs increasing doses of Doxorubicin (Doxo) over 6 days (scale bar = 500 µm). (D) Representative contraction amplitude plots of Long-term 3D hOCMTs in response to untreated vs increasing doses of Doxo over 6 days (t = 15 s). (E) Average beating rates of Long-term 3D hOCMTs in response untreated vs increasing doses of Doxo over 6 days (n = 6, Lines = Median ± min/max, Dots = Mean value, Dunnett’s multiple comparisons test). (F) Luminescence-based quantification of cell viability Long-term 3D hOCMTs on day 6 of Doxo treatment normalized to untreated samples (untreated vs 0.1 µg/mL vs 1 µg/mL) (n = 6, Mean ± SD, Tukey’s multiple comparisons test). (G) Representative cell viability of v on day 6 of Doxo treatment (untreated vs 0.1 µg/mL vs 1 µg/mL) with Calcein/AM staining, green = live, red = dead (Scale bar = 100 µm).
