Figure 1

Genotyping schematic used for the generation of Müller-glial-cell-specific Il6ra−/− mice. A representative agarose gel shows genotyping PCR products for Cre (635 bp) and floxed (123 bp) or wildtype (WT, 109 bp) Il6ra alleles in mice from F0–F3 generations. Genotyping results are shown for F0 (fl/fl and wt/wt Cre+), F1 (wt/fl and wt/fl Cre+), F2 (wt/fl, fl/fl, wt/fl Cre+, and fl/fl Cre + KO), and F3 (fl/fl Cre + KO); M, GeneRuler 1 kb Plus DNA Ladder.