Figure 2 | Scientific Reports

Figure 2

From: Generation and characterization of a Müller-glial-cell-specific Il6ra knockout mouse to delineate the effects of IL-6 trans-signaling in the retina

Figure 2

RT-PCR confirmation of Il6ra−/− in isolated Müller glial cells (MGCs). (A) Overview of the breeding strategy used to generate MGC-specific Il6ra−/− mice. (B) Relative Il6ra mRNA expression in isolated knockout MGCs (KO) compared to wildtype (WT) by RT-PCR using primers specific to the junction of exons 2 and 3 (E2–E3, blue), exons 5 and 6 (E5–E6, red), and within exon 10 (E10, green). Data is normalized to Gapdh expression (2−ΔΔCq) and reported as mean fold control (KO vs. WT) ± SD, n = 6–7/group, ****p-value < 0.0001 vs. WT (Two-way ANOVA, Tukey’s multiple comparisons test). (C) RT-PCR confirmation of Cre mRNA expression in KO MGCs normalized to Gapdh. As expected, Cre mRNA expression was not detected (ND) in WT MGCs. Data is represented as mean ± SD, n = 5/group; statistical significance was not calculated due to no detection in WT.

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