Figure 4

Immunofluorescence staining confirms a Müller-glial-cell-specific knockout of Il6ra in the retina. Representative images of MGC-specific Il6ra KO and wildtype (WT) mouse retinal sections stained with H&E and immunofluorescence for IL-6Rα (green) and GLAST (red), a marker of Müller glia, showing colocalization of GLAST and IL-6Rα (yellow). Data is representative of images acquired using a Keyence microscope (H&E) at 20× magnification and a Leica STELLARIS Confocal Microscope (immunofluorescence staining) at 63× magnification.