Table 1 Summary of UPLC-MS/MS assays.
From: Suppressed renoprotective purines in COVID-19 patients with acute kidney injury
Internal standard or Target purine | Vendor | Precursor ion (m/z) | Collision energy (volts) | Product ion (m/z) | Approximate retention time (min) |
|---|---|---|---|---|---|
Analysis of adenosine | |||||
13C10-Adenosine | Medical Isotopes, Inc. | 278 | 19 | 141 | 3.18 |
Adenosine | Sigma-Aldrich | 268 | 19 | 136 | 3.18 |
Analysis of 3’,5’-cAMP | |||||
13C5-3’,5’-cAMP | Toronto Research Chemicals | 335 | 28 | 136 | 3.23 |
3’,5’-cAMP | Sigma-Aldrich | 330 | 28 | 136 | 3.23 |
Analysis of 5’-AMP | |||||
13C10-5’-AMP | Medical Isotopes, Inc. | 358 | 19 | 141 | 1.60 |
5’-AMP | Sigma-Aldrich | 348 | 19 | 136 | 1.60 |
Analysis of inosine | |||||
15N4-Inosine | Cambridge Isotope Laboratories | 273 | 20 | 141 | 2.92 |
Inosine | Sigma-Aldrich | 269 | 20 | 137 | 2.92 |
Analysis of hypoxanthine | |||||
13C5-Hypoxanthine | Cambridge Isotope Laboratories | 141.8 | 22 | 124 | 1.77 |
Hypoxanthine | Sigma-Aldrich | 136.8 | 22 | 119 | 1.77 |
Analysis of xanthine | |||||
15N2-Xanthine | Cambridge Isotope Laboratories | 154.9 | 20 | 137.8 | 1.89 |
Xanthine | Sigma-Aldrich | 152.9 | 20 | 135.8 | 1.89 |
Analysis of guanosine | |||||
13C10,15N5-Guanosine | Medical Isotopes, Inc. | 299 | 20 | 162 | 2.94 |
Guanosine | Sigma-Aldrich | 284 | 20 | 152 | 2.94 |
Analysis of 3’,5’-cGMP | |||||
13C5-3’,5’-cGMP | Toronto Research Chemicals | 351 | 16 | 152 | 3.23 |
3’,5’-cGMP | Sigma-Aldrich | 346 | 16 | 152 | 3.23 |
Analysis of 5’-GMP | |||||
13C10-5’-GMP | Medical Isotopes, Inc. | 374 | 15 | 157 | 1.60 |
5’-GMP | Sigma-Aldrich | 364 | 15 | 152 | 1.60 |
Analysis of guanine | |||||
13C2,15N-Guanine | Medical Isotopes, Inc. | 155 | 20 | 138 | 1.48 |
Guanine | Sigma-Aldrich | 152 | 20 | 135 | 1.48 |
Analysis of 8-aminoguanine | |||||
13C2,15N-8-Aminoguanine | Medical Isotopes, Inc. | 170 | 18 | 153 | 1.43 |
8-Aminoguanine | Toronto Research Chemicals | 167 | 18 | 150 | 1.43 |
