Table 2 Expression stability M-value of 12 candidate reference genes calculated by geNorm.

From: Validation of suitable reference genes by various algorithms for gene expression analysis in Isodon rubescens under different abiotic stresses

Stability rank

Total

DT

SA

MeJA

ABA

Dehydration

NaCl

ND

SMA

Gene

Values

Gene

Values

Gene

Values

Gene

Values

Gene

Values

Gene

Values

Gene

Values

Gene

Values

Gene

Values

1

ACT/GADPH

0.6187

18S/ eIF

0.2826

18S/eIF

0.297

UBQ/eIF

0.288

Cyc1/PP2A

0.213

Apt/ eIF

0.414

TUB/ACT

0.191

HIS/UBQ

0.2465

ACT/GADPH

0.553

2

Apt

0.8555

GADPH

0.526

GADPH

0.496

Apt

0.342

UBQ

0.371

UBQ

0.466

Cyc1

0.379

Apt

0.4579

Cycl

0.6911

3

UBQ

0.9261

TUA

0.6244

TUA

0.58

PP2A

0.389

HIS

0.446

HIS

0.522

PP2A

0.405

PP2A

0.5109

UBQ

0.7893

4

eIF

0.9903

ACT

0.6961

ACT

0.645

Cyc1

0.439

EF-1α

0.495

TUA

0.549

UBQ

0.431

TUA

0.547

Apt

0.8151

5

HIS

1.0642

Apt

0.7242

Apt

0.678

GADPH

0.478

TUB

0.547

PP2A

0.593

HIS

0.46

eIF

0.5977

eIF

0.8656

6

18S

1.1069

EF-1α

0.7949

TUB

0.752

ACT

0.585

ACT

0.621

GADPH

0.652

EF-1α

0.479

GADPH

0.6296

18S

0.9476

7

TUB

1.1385

TUB

0.8317

EF-1α

0.802

18S

0.691

Apt

0.683

18S

0.746

Apt

0.524

EF-1α

0.704

PP2A

1.0321

8

PP2A

1.1767

HIS

0.8929

HIS

0.852

HIS

0.769

eIF

0.722

ACT

0.809

eIF

0.566

Cycl

0.7464

HIS

1.072

9

TUA

1.2512

PP2A

1.0026

PP2A

0.953

TUB

0.841

GADPH

0.753

EF-1α

0.888

GADPH

0.592

ACT

0.7871

TUB

1.1157

10

Cycl

1.3097

UBQ

1.1452

UBQ

1.077

EF-1α

0.888

18S

0.819

TUB

0.978

TUA

0.617

TUB

0.8313

TUA

1.2041

11

EF-1α

1.4472

Cycl

1.3525

Cyc1

1.263

TUA

1.034

TUA

0.874

Cyc1

1.096

18S

0.683

18S

0.8707

EF-1α

1.3568