Figure 1 | Scientific Reports

Figure 1

From: Enhanced CHOLESTEROL biosynthesis promotes breast cancer metastasis via modulating CCDC25 expression and neutrophil extracellular traps formation

Figure 1

Cholesterol biosynthesis enhances colony and sphere formation in breast cancer cells. 4T1 (a) and MDA-MB-231 (b) cells were infected with lentivirus expressing shRNA targeting ASPP2 or HMGCR, and scramble shRNA (shNon) as control. 2 μM Simvastatin or 10 μM BBR were added to LV-shASPP2 infected cells. 24 h later, cells were collected and used for cholesterol content assays. (c) 2 × 103 4T1 cells per well were cultured in 6-well plates for 24 h, then cultured in medium containing 20% FBS for 12-15d. Colony number and representative images (left) were taken. (d) 1 × 105 4T1 cells were cultured onto ultralow attachment plates. Number of sphere formation in primary (6d) and secondary spheres (14d) were calculated. Representative images (left) was shown. Scale bar: 50 µm. (e) qRT-PCR assay for CD133, CD44, EpCAM and OCT-4 mRNA levels in treated 4T1 cells. *P < 0.05; **P < 0.01; ***P < 0.001.

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