Figure 2 | Scientific Reports

Figure 2

From: Enhanced CHOLESTEROL biosynthesis promotes breast cancer metastasis via modulating CCDC25 expression and neutrophil extracellular traps formation

Figure 2

Cholesterol biosynthesis promotes cancer cells invasive abilities and formation of NETs in vitro. (a) 4T1 and MDA-MB-231 cells cultured with Matrigel in the upper chamber of the Transwells. 10 μM BBR, 2 μM simvastatin or DMSO was added to shASPP2-infected cancer cells. PMA stimulate neutrophil in serum-free medium with or without DNase I were add to the bottom wells. After cultured 22–26 h, counted invading cells by a light Microscope. Invasion was calculated by compared with invading cells in shNon group. Scale bar: 200 μm. (b,c) NETs formation as detected by immunofluorescence staining for H3cit and MPO were analyzed on neutrophils-cultured slides with 4T1 cells (b) or MDA-MB-231 cells (c). The areas marked by the white boxes are shown magnified in the insets in the top right. Scale bar: 50 μm. (d) SytoxGreen stainings were performed on neutrophils-cultured slides with MDA-MB-231 cells. Scale bar: 50 μm. *P < 0.05; **P < 0.01;***P < 0.001.

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