Figure 3

(A) A representative microscopic image of Ki67 expression in adenomyosis lesion (× 400) and a box and whisker graph of the percentage of Ki67 positive cells Ki67 is positive in the nuclei of both epithelial and stromal cells (arrowheads) in the adenomyosis lesion at the 14th (D14) (left) and the 65th (D65) (right) postoperative day. In epithelial cells, the percentage of Ki67 positive cells was significantly higher in D14 than in D65 (p < 0.05), while in stromal cells, the percentage was significantly higher in D65 than in D14 (p < 0.01). (B) A representative microscopic image of CD31 expression in adenomyosis lesion (×400) and a box and whisker graph of vascular density. CD31, a marker for endothelial cells, was used to detect blood vessels. Blood vessels within the lesion were identified, the cross-sectional area of blood vessels was measured, and this was divided by the cross-sectional area of adenomyosis lesion to calculate the vascular density. Vascular density in D65 was significantly higher than that in D14 (p < 0.05). (C) A representative microscopic image of Masson’s trichrome staining of adenomyosis lesion (×400) and a box and whisker graph of percentage of fibrotic area. Areas of fibrosis (collagen deposition) were stained blue. The percentage of fibrosis area in D65 was significantly higher than that in D14 (p < 0.001). Box: 25–75%, whisker: 10–90%, midline: median, *p < 0.05, **p < 0.01, ****p < 0.001.