Table 3 Primers used for the amplification and sequencing of the ssrDNA of the new Henneguya spp.

From: Diversity of myxozoans parasitizing the catfish Rhamdia quelen (Siluriformes: Heptapteridae), in southeastern Brazil, based on morphological and molecular evidence

Primer

Sequence 5′–3′

Paired with

Reference

Erib1

ACCTGGTTGATCCTGCCAG

Act1r

Barta et al.39

Myxgen4F

GTGCCTTGAATAAATCAGAG

Erib10

Kent et al.40

Act1r

AATTTCACCTCTCGCTGCCA

Erib1

Hallett and Diamant41

Erib10

CTTCCGCAGGTTCACCTACGG

Myxgen4F

Barta et al.39

MB5

GGTGATGATTAACAGGAGCGGT

MX3

Eszterbauer42

MX3

CCAGGACATCTTAGGGCATCACAGA

MB5

Andree et al.32

  1. Cycling conditions (for all primers): Initial denaturation at 95 °C for 3 min, followed by 35 cycles of 95 °C for 1 min, annealing at 55 °C for 45 s, 72 °C for 2 min, and a final extension at 72 °C for 7 min.
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