Figure 2 | Scientific Reports

Figure 2

From: A mouse model of hepatic encephalopathy: bile duct ligation induces brain ammonia overload, glial cell activation and neuroinflammation

Figure 2

Evolution of liver disease in C57Bl/6j and Swiss mice after chronic BDL. C57Bl/6j mice were subjected to sham or BDL for 7, 14, 21 and 28 days. Swiss mice were subjected to sham or BDL for 6 and 8 weeks. (A) Plasma levels of AST, ALT, bilirubin, albumin and ammonia at different timepoints after sham (C57Bl/6j mice: n = 5–15/timepoint; Swiss mice: n = 13) or BDL (C57Bl/6j mice: n = 6–9/timepoint; Swiss mice: n = 5–13/timepoint) induction. (B) Representative H&E and Sirius Red stained images after sham and BDL induction. Quantification of the fibrotic area on Sirius Red stained liver sections of sham (C57Bl/6j mice: n = 5/timepoint; Swiss mice: n = 7) or BDL (C57Bl/6j mice: n = 5/timepoint; Swiss mice: n = 13) mice at different timepoints. Quantification of biliary infarcts on H&E-stained liver sections of sham (C57Bl/6j mice: n = 5/timepoint; Swiss mice: n = 7) or BDL (C57Bl/6j mice: n = 5/timepoint; Swiss mice: n = 11) mice at different timepoints. Data are represented as means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. sham control. Arrowheads indicate biliary infarcts. Scale bars represent 200 µm. BDL bile duct ligation.

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