Figure 10

Quantification of MbxA-induced permeabilization of HEp-2 cells. Mean Sytox Green intensity per nucleus of HEp-2 cells (y-axis) was continuously measured over a period of 20 min at 37 °C (see mateirals and methods for details) and is plotted against time (x-axis) for different MbxA concentrations (250 nM, 30 nM and 10 nM) and 250 nM. Sytox Green staining of the HEp-2 cells´ DNA is faster and more intense when 250 nM MbxA were used and is reduced at lower MbxA concentrations (30 nM and 10 nM). In contrast to that, no significant Sytox Green staining of the nuclear DNA was observed with 250 nM proMbxA (number of nuclei used for quantification: 250 nM MbxA—71, 30 nM MbxA—74, 10 nM MbxA—79, 250 nM proMbxA—62). Nuclei have been analyzed from two independent experiments with two biological replicates each. The original images at different time points are provided in Fig. S7.