Figure 11
Incubation experiment of Atto488-MbxAS9C with HEp-2 cells. HEp-2 cells after 30 min of incubation with 250 nM Atto488-MbxAS9C, before (a and b) and after (d and e) removing excess unbound Atto488-MbxAS9C. Confocal overview images of fluorescent signal are shown in (a) and (d), transmitted light showing formation of blebbing process in (b) and super-resolution Airyscan micrograph in (e). Accumulations of Atto488-MbxAS9C tracing the outline of HEp-2 cells are clearly visible after removing the unbound Atto488-MbxAS9C (d). Control experiment with Atto488 (dye used to label MbxA) is shown in (c) and (f). HEp-2 cells after 30 min of incubation with Atto488, before (c) and after (f) washing off the unbound Atto488 from surrounding medium. The control experiment confirmed that accumulations tracing the outline of HEp-2 cells in (d) and (e) are Atto488-MbxAS9C, not the free dye. White arrows indicate the position of membrane blebs. (g) Zoom into white square in panel (d). Scale bars: a, b, c, d, f: 50 µm; e: 10 µm.
