Figure 2
From: iPSC-neural crest derived cells embedded in 3D printable bio-ink promote cranial bone defect repair

3D printed constructs for bone regeneration. 3D printed Ink-Bone construct can be designed and printed with or without cells. (A) Design of 3D printed construct. (B)–(D) Characterization of 3D printed constructs. (B) Optical image, (C) SEM from top view, and (D) SEM from cross-section of 3D printed scaffold without cells. (E) Optical image of 3D printed scaffold with cells (9 m/ml). The uCT images of 3D printed constructs (F) without cells and (G) with 9 m/ml cells and cultured after 27 days. In (A), the scaffold design is 10 mm × 10 mm × 2 mm square. Printed at 10 mm/s. Air pressure is 25kpa for (B) and (F), and 30kpa for (C)–(E) and (G). The original magnifications for (C) and (D) are ×50 and ×70 respectively.