Figure 3
From: iPSC-neural crest derived cells embedded in 3D printable bio-ink promote cranial bone defect repair
Cell viability and in vitro bone formation increased in the Ink-bone group. A. Cells were labeled with a Luciferase reporter gene and their viability in the two bio inks was assessed by BLI in vitro. N ≥ 4. B. Cells were cultured in 2D or in bio-ink 3D constructs in osteogenic media. Osteogenic gene expression was tested on Day 10. N ≥ 4. C. In vitro bone formation of cells in the bio-inks was evaluated using µCT at 3 weeks and compared to Ink-Bone only. N = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
