Figure 1 | Scientific Reports

Figure 1

From: Establishment of in vivo proximity labeling with biotin using TurboID in the filamentous fungus Sordaria macrospora

Figure 1

Schematic illustration of constructs used in BioID experiments. For BioID experiments, multiple constructs for the SCI1-TurboID fusion protein and the unfused TurboID control have been generated. The TurboID sequence was codon-optimized according to the codon usage table of S. macrospora18. Expression is controlled by different promoters: p5’, native sci1 promoter; pc, promoter of the clock-controlled gene 1 (ccg1) from N. crassa for overexpression; px, xylose inducible promoter (Smxyl) of the beta-xylanase gene (SMAC_08023) from S. macrospora19; L, 11 amino acid linker (MGGGGSGGGGS) attached to N-terminus of TurboID; TtrpC, terminator of the anthranilate synthase gene from A. nidulans; a scalebar is indicated.

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