Table 6 Effects of milk feeding level (medium vs. high) and milk feeding frequency (2 vs. 3) on first breeding and first lactation of heifers.

Item	Treatments¹				SEM	P-value²
Item	ML-2 ×	ML-3 ×	HL-2 ×	HL-3 ×	SEM	L	F	L*F
First breeding performance
Wither height at AI³, cm	131	133	132	133	1.21	0.50	0.23	0.45
Weight at AI, kg	356	350	366	365	6.91	0.09	0.64	0.69
Age at first AI, d	384	382	377	375	3.70	0.05	0.51	0.88
Age at first calving, month	22.3	23.9	23.7	23.5	1.02	0.57	0.48	0.34
Service per conception (first parity)	2.0	1.8	1.8	2.4	0.56	0.96	0.72	0.50
Milk yield and milk quality, kg
Milk 305 d, kg	10,350	10,747	11,127	12,360	556	0.07	0.20	0.51
ECM 305 d, kg	9435	10,007	10,129	11,418	593	0.09	0.13	0.55
Fat 305 d, kg	307	320	329	401	34.8	0.20	0.29	0.46
Protein 305 d, kg	289	326	309	304	16.3	0.94	0.33	0.21
Lactation length, d	396	343	304	308	53.6	0.19	0.53	0.87

¹Treatments including medium milk level- feeding 2 times (ML-2 ×), medium milk level- feeding 3 times (ML-3 ×), high milk level- feeding 2 times (HL-2 ×), and high milk level- feeding 3 times (HL-3 ×).
²Statistical comparisons: L = feeding level; F = feeding frequency, L × F = feeding level × feeding frequency.
³AI = Artificial insemination.

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