Table 12 Analysis of parameters based quantitative cycling points (CP) for 10 candidate RGs in DHC.

From: Selection of species specific panel of reference genes in peripheral blood mononuclear cells of native livestock species adapted to trans-Himalayan region of Leh-Ladakh

 

GAPDH

ACTB

RPS9

EEF1A1

RPS15

RPS23

UXT

RPL4

B2M

HPRT1

n

5

5

5

5

5

5

5

5

5

5

geo Mean [CP]

22.53

19.15

19.57

17.38

32.37

17.04

19.66

20.31

20.41

22.27

AR Mean [CP]

22.57

19.16

19.58

17.39

32.42

17.05

19.68

20.38

20.42

22.27

min [CP]

20.41

18.37

18.55

16.87

30.62

16.05

18.19

18.23

19.24

21.93

max [CP]

24.13

20

20.83

17.93

34.9

17.98

20.89

22.32

21.4

22.78

std dev [+/− CP]

0.86

0.38

0.54

0.29

1.61

0.46

0.69

1.54

0.57

0.2

CV [% CP]

3.82

1.97

2.77

1.69

4.97

2.71

3.52

7.55

2.8

0.91

min [x-fold]

− 4.35

− 1.72

− 2.03

− 1.43

− 3.37

− 1.98

− 2.76

− 4.23

− 2.25

− 1.27

max [x-fold]

3.03

1.8

2.4

1.46

5.77

1.92

2.35

4.03

1.99

1.42

std dev [+ /− x-fold]

1.82

1.3

1.46

1.23

3.05

1.38

1.62

2.9

1.49

1.15