Figure 5 | Scientific Reports

Figure 5

From: Co-aggregation of MSC/chondrocyte in a dynamic 3D culture elevates the therapeutic effect of secreted extracellular vesicles on osteoarthritis in a rat model

Figure 5

(A, a) Radiological photograph from lateral and ventrodorsal view (VD) of rats and (A, b) their statistical analysis. (B, a) In vitro histological analysis; knee sections were stained using Hematoxylin and Eosin (H&E), Toluidine blue (TB) Safranin O (SO) and immunohistochemical staining for COL II. HC: hyaline cartilage, SB: subchondral bone, FT: fibrous tissue, black arrows: the destruction of articular cartilage, yellow arrow: superficial cleft of articular cartilage, red arrow: thickened articular surface + chondrocytes depletion, green arrows: micro-lesions in articular cartilage surface (scale bars = 100 μm). (B, b) Mankin’s OA score statistical analysis, (B, c) DAB signaling (%) 5ROI statistical analysis for COL II. The results are expressed as the mean ± SEM. *Significant difference compared with the OA ctrl (*p < 0.05;  **p < 0.01;  ***p < 0.001; and ****p < 0.0001).

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