Figure 1

Scanning electron microscopy and PCR analysis of mouse feces. (A) PCR analysis of fecal DNA isolated from conventional, germ-free, and gut-colonized (Env MT, mouse FMT and human FMT) mice using bacterial universal and turicibacter primers. The absence of PCR amplicons is indicative of the lack of bacterial gut colonization in germ-free B6 mice. Detection of PCR amplicons in gut-colonized mice is suggestive of efficient gut microbial colonization at levels similar to that of conventional B6 mice. The samples were loaded in the sequential order and the unloaded wells were cropped from the image. RC# represents number given to individual animal. (B) Representative SEM images of fecal samples from germ-free, conventional and mouse FMT mice. Scale bars 10 µM—2 mm. No bacteria are found in the feces of germ-free mice. Different types of bacteria are found in the feces of conventional and mouse FMT mice.