Figure 3
From: Genesis of fecal floatation is causally linked to gut microbial colonization in mice
Quantitative flow cytometry analysis of fecal microbial dynamics. (A) Overlayed contour FACS plots of conventional and human FMT samples showing 2 distinct clusters of microbial flora in the upper half of the plot. The human FMT group shows presence of only 1 of the 2 clusters seen in the conventional mice samples. Arrows indicate microbial presence, UFP and beads. (B) Representation of gates drawn for microorganisms and counting beads using flow cytometry analysis. (C) Flow cytometry plots of 3 fecal samples from each group depicting lack of distinct microbial cluster and enrichment of fecal UFP in germ-free feces. These plots also highlight differing levels of microbial and UFP particles in feces. It is notable that even in germ-free feces, low background counts are consistently observed which may be due to particles introduced from feces and sample processing steps. (D) Pearson’s (non-linear regression) correlation between the fecal UFP and the fecal microbial counts for all fecal samples. The outlier in data could represent occasional disrupted relationship between the microbial density and UFP. (E) Absolute microbial counts per mg feces.
