Figure 3

Venn diagram showing bacterial genera unique to and shared between the examined sites along the Białka river. Values in petals indicate from the top: numbers of genera in each group; proportions of the overall number of genera in each group (in round brackets), and proportions of 16S sequences belonging to unique genera in each group (in square brackets). Arrow graph shows the expected bacterial pollution gradient, darker brown shade indicates the more contaminated sites, the arrowhead indicates the course of Białka river. Please note the diversity increase similar to the pollution gradient, i.e. GW < TNP = DSTP2 < USTP = DSTP1 < STP, expressed by high numbers / proportion of unique genera, more diverse composition of bacterial genera in more polluted sites. Site abbreviations are as follows: GW—groundwater; TNP – Tatra National Park; USTP—upstream of the sewage treatment plant; STP—sewage treatment plant; DSTP1—c.a. 3 km downstream of the STP; DSTP2—c.a. 7 km downstream of the STP (for detailed description, please see caption of Fig. 2).