Figure 2
From: A simple label-free method reveals bacterial growth dynamics and antibiotic action in real-time
Bacteria of each species were resuscitated from glycerol stock and inoculated into Mueller–Hinton cation adjusted (MH+) media (Sigma) and allowed to incubate overnight (~ 16 h). Seven serial 1:10 dilutions with 100 µL of culture in 900 µL of MH+ media in sterile microcentrifuge tubes (Axel, Germany) were carried out. Each serial dilution was aliquoted into sterile cuvettes, capped and measured in a spectrophotometer (Biochrom WPA CO8000, UK) using sterile MH+ media as a blank. All experiments were performed in triplicate to provide the sensitivity of the spectrophotometer. The same samples were transferred to the SLIC v7 device and scattering signal acquired over 10 s. The average of the three data points were collected. The cuvettes were removed from the SLIC device and for each 3 × 10 µL was aliquoted onto a MH+ agar plate and the number of viable organisms determined by a modified Miles and Misra7. Mean values for both parameters were plotted together with the standard error of the mean.
