Figure 1 | Scientific Reports

Figure 1

From: Differential expression and regulation of HSP70 gene during growth phase in ruminants in response to heat stress

Figure 1

Relative mRNA expression (fold change) of HSP70 gene in different age groups of Jamunapari goat. 3 M, 3-month age of animal; 9 M, 9-month age of animal; 12 M, 12-month age of animal; adult, 2–3 year age of animals. The crossing point (Cp) readings for each unknown sample were then used to calculate the amount of either the target or housekeeping gene using the second derivative maximum method with the Light cycler 480 analysis software version 1.5 (Roche Applied Science, Indianapolis, IL, USA). GADPH and β-actin were used to normalize gene expression. The susceptible individual was used as a positive calibrator to obtain normalized gene expression.

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