Figure 2

Glycoblotting-based microarray for galectin interaction profiling with core M1 modification. Synthetic α-DG core M1 glycoconjugates used for microarray experiment (a). Compounds were robotically printed in quadruplets at 20 and/or 200 μM on an aminooxy- coated plastic slide. Subsequently, a six-chamber rubber silicon sheet was attached. Green spots correspond to cyanine 3-keto-BSA (Cy3-keto-BSA) as grid (b).