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Figure 1

From: Optimising expression of the large dynamic range FRET pair mNeonGreen and superfolder mTurquoise2ox for use in the Escherichia coli cytoplasm

Figure 1

N-terminally optimised sfTq2oxopt is expressed better than sfTq2ox. (A) Microscopy images, Phase-contrast images are shown on the left, with the corresponding fluorescence images on the right. The numbers above the fluorescence images are the brightness and contrast settings for the image. Scale bar equals 2 µm; (B) Graphic representation of the constructs tested in this panel; (C) Fluorescence per µm3 cell, based on microscopy of 650–770 cells, the bar represents mean value and error bars standard deviation; (D) Western blot detecting sfTq2ox(opt) with αGFP reveals higher expression levels of unfused sfTq2oxopt and sfTq2oxopt-FtsZ compared to unfused and FtsZ-fused sfTq2ox. On the contrary, the fusion to the C-terminus of OmpA1-177 did not cause increased expression. Original blots shown in Supplementary Fig. S2; E. Alignment of sequences of sfTq2ox and sfTq2oxopt, mutations are based on gfp-mut240, shown with grey highlighting.

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