Figure 3 | Scientific Reports

Figure 3

From: Optimising expression of the large dynamic range FRET pair mNeonGreen and superfolder mTurquoise2ox for use in the Escherichia coli cytoplasm

Figure 3

mNG-v5 contains an alternative RBS, and its start of translation is predominantly Met10. (A) Fluorescence per µm3 cell for different versions of mNG and RBSs of mNG, based on microscopy of two independent experiments, represented by triangles, 185–666 cells per sample were analysed. The bars represent mean value. Representative microscopy images can be found in Supplementary Fig. S1; (B) Sequence of the three different 5′ UTRs to Met10 of three different mNG-v4 constructs. Introduced nucleotides have been highlighted in red, and putative RBSs are underlined.

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