Figure 4 | Scientific Reports

Figure 4

From: Optimising expression of the large dynamic range FRET pair mNeonGreen and superfolder mTurquoise2ox for use in the Escherichia coli cytoplasm

Figure 4

sfTq2oxopt and RBSshort-mNG-v4 form a balanced FRET pair for the cytoplasm. (A) Schematic drawing of the inner membrane with fluorescent fusions to PBP2, RodA and GlpT. The curved arrow represents FRET; dashed arrow indicates that the distance between non-interacting proteins is too large to measure FRET; (B) EfA (%) detected for tandem (RBSshort-mNG-v4-sfTq2ox), negative control (RBSshort-mNG-v4-RodA and sfTq2oxopt-GlpT) and biological interaction between RBSshort-mNG-v4-RodA and sfTq2oxopt-PBP2. The bar represents mean value and error bars standard deviation of four replicates. Supplementary Table S3 shows the actual values for EfA. Representative microscopy images can be found in Supplementary Fig. S6 and the unmixing of the individual FRET experiments in Supplementary Fig. S7.

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