Figure 2 | Scientific Reports

Figure 2

From: Assessment of the biofilm-forming ability on solid surfaces of periprosthetic infection-associated pathogens

Figure 2The alternative text for this image may have been generated using AI.

Biofilm formation on polystyrene (PS) surface. Each microorganism was cultured in PS tubes for 24, 48, or 72 h. Culture tubes and their supernatants were collected separately at each time point. (a) The culture tubes with biofilm were stained with crystal violet (CV), and their absorbance was measured at 550 nm to assess biofilm formation on the surface. (b) The pellets of planktonic cells in each supernatant were separately stained with CV. Their absorbances were measured at 550 nm to assess the amounts of planktonic cells in the tube. (c) The optical density (OD) of non-adherent planktonic cell-containing supernatants was measured at 600 nm. (d) The OD values obtained from the CV assay for biofilm formation were divided by the sum of OD values from the CV assay for biofilm formation on the surface and from planktonic cell-containing supernatant to calculate the ratio of biofilm formation.

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