Table 7 Fractional inhibition of hDHODH, combination index values (CI) and DRI values of silymarin standard extract and brequinar in combination at several effect levels.

From: Discovery of potential natural dihydroorotate dehydrogenase inhibitors and their synergism with brequinar via integrated molecular docking, dynamic simulations and in vitro approach

(Fa × 100)

% (hDHODH) Inhibition of the combined drugs (%)

CI

values

Dose (ug/ml) Silymarin standard extract

Dose (nM)

Brequinar

DRI

Silymarin standard extract

DRI

Brequinar

17

1.33

(Ant)

47.76

2.92

1.46

1.55

33

1.79

(Ant)

85.75

5.81

1.10

1.13

72

1.03

(Add)

245.88

19.99

1.84

2.05

83

1.14

(Ant)

373.37

32.64

1.63

1.77

98

0.55

(Syn)

1805.11

207.41

3.32

4.08

  1. Fa signifies fraction affected. CI < 1, = 1, and > 1 indicate synergism (Syn), additive effect (Add), and antagonism (Ant), respectively. DRI > 1 indicates favourable dose reduction (in fold) for the drug in combination.
Back to article page